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Basic Research Skills Seminar (8 Feb 2017)


Basic Research Skills Seminar

Centre for Genomic Sciences

We are pleased to announce this new series of seminars aimed at equipping researchers who are doing genetics-related research with appropriate skills in statistics, computing, and bioinformatics.

Analysis of RNA-seq Data

Dr Peikai Chen

8 Feb 2017 (Wed) 3:00 – 4:00 pm

Seminar Room 2, G/F
The Hong Kong Jockey Club Building for Interdisciplinary Research
 5 Sassoon Road, Pokfulam, Hong Kong


The transcriptome of biological systems acts as an intermediate process for cellular functionalities and their regulations. The various types of RNAs play important yet different roles, on a time- and location-specific manner. The measurement of RNA expression activities has evolved over the years. Current RNA-seq technology is highly popular for its capacity to perform massive, unbiased, de-novo, high-resolution, and economic measurements of the transcriptome. Lately, RNA-seq is even more exciting due to its use in measuring single cell transcriptomes. There are several standard pipelines to process RNAseq data. However, their versatility coupled with complex technical issues causes significant variations in data and results. In this seminar, I will introduce the basics in the processing of RNAseq data, including aligning, assembling, quantifying, comparing across different conditions, and gene set analyses. More advanced topics, such as de novo assembly for intergenic RNAs and single-cell RNAseq analyses, will also be briefly discussed.

Other seminars in the series:

Basic Statistics/Probability Theory for Geneticists by Dr Timothy Mak
13 Feb 2017 (Mon) 3:00-4:00pm

Applications of Regression and Correlation in Genetics by Dr Timothy Mak
20 Feb 2017 (Mon) 3:00-4:00pm

Analysis of Rare Variants Association by Mr Robert Porsch
7 Mar 2017 (Tue) 2:30-3:30pm



For enquiries, please call 2831-5096 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it