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Illumina iScan SystemiScan BeadArray Technology Overview

Illumina iScan System utilizes BeadArray technology for a broad range of DNA and RNA analysis applications including genotyping, gene expression and methylation studies. The 3-micron silica beads are assembled in microwells on slide with high density, enabling multi-sample per slide. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in one of Illumina’s assays. Different combinations of probe design enable a wide range of applications on a whole-genome scale, including gene- and exon-level expression analysis, novel transcript discovery, genotyping, and resequencing.

The iScan platform can perform the following analysis:

  • Whole genome SNP genotyping
  • Whole genome methylation analysis
  • Whole genome copy number variation (CNV) analysis
  • Custom SNP genotyping
  • Gene Expression profiling

A typical iScan BeadChip experiment (Infinium) includes the following steps:

  • Preparation of DNA isolated from specimens
  • Hybridization of sample to BeadChips
  • Stringent washes, single base extension, staining and imaging of BeadChips
  • Analysis of scanned images and data extraction

For details of iScan BeadArrayTechnology and products, please visit Illumina iScan website.

 

Contact

  Ms Rachel Li
  Ms Levina Lam
  Mr Carlos Choi
  Ms Tiky Leung
  Ms Rebecca Ho

  Telephone: 2831 5467 / 2831 5477
  Email: iscan.cgs@hku.hk