Home Nucleic Acids Extraction

Technology Overview

Print

EZ1 Advanced XLEZ1 Advanced XL is a mid-throughput, fully automated nucleic acid extraction system.

Nucleic acids are isolated from lysates in one step through binding to the silica surface of magnetic particles in the presence of a chaotropic salt. The particles are then separated from the lysates with magnetic force.  After washing, pure DNA or RNA is eluted.

Fully automated procedure offers consistent sample processing and minimizes potential variations introduced by manual labour.

Nine different DNA extraction protocols optimized for various sample types are currently available at the Centre for Genomic Sciences:

Combinations of EZ1 Advanced Cards and EZ1 Kits:

Protocol Card Reagent Kit Sample Type
DNA Blood DNA Blood Kit Blood and blood-derived samples
DNA Buffy Coat DNA Blood Kit Buffy coat
DNA Investigator DNA Investigator Kit Forensic and human identity samples
DNA Tissue DNA Tissue Kit Tissues
DNA Bacteria DNA Tissue Kit Human samples, swabs, biopsies, bacterial cultures
DNA Dried Blood DNA Tissue Kit Dried blood
DNA Paraffin Section DNA Tissue Kit Paraffin-embedded tissues
DNA Buccal Swab DNA Tissue Kit Buccal swabs
Virus Card v 2.0 Virus Mini Kit v2.0 Viral DNA and RNA from serum, plasma, CSF, urine, and respiratory samples

Purified nucleic acids are ready for use in downstream applications e.g. MassArray genotyping and Sanger DNA sequencing.