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Technology Overview

Pacific Biosystems Sequel

PacBio sequencing is a high throughput / third-generation sequencing technology which also known as single molecule real time (SMRT) sequencing.  It utilizes zero-mode waveguides (ZMWs) to capture the real-time incorporation of nucleotides by DNA polymerase.  This technology consistently produces long reads (average > 10,000 bp) which enables assembly of high-quality de novo genomes.  It is also good for the survey of full-length transcript isoforms.  Barcoding system is available for multiplexed sequencing on a single SMRT cell to achieve time- and cost- effectiveness.

The PacBio Sequel system has been installed at CGS in June 2017.  Protocols are being actively developed.  Please contact us if you are interested!



Sample Type Service Type
DNA   Whole Genome Sequencing
DNA   Microbial Genome Sequencing
DNA   Targeted Sequencing
DNA   Metagenomic Sequencing
RNA   RNA Sequencing (Iso-seq)


  Mr Chengwei Wu
  Ms Tiky Leung
  Mr Clement Chow
  Mr Allen Chu

  Telephone: 2831 5478 / 2831 5477
  Email: thirdgenseq.cgs@hku.hk