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Technology Overview

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Pacific Biosystems Sequel

Pacbio sequencing is a high throughput / third-generation sequencing technology which also known as single molecule real time (SMRT) sequencing.  It utilizes zero-mode waveguides (ZMWs) to capture the real-time incorporation of nucleotides by DNA polymerase.  This technology consistently produces long reads (average > 10,000 bp) which enables assembly of high-quality de novo genomes.  It is also good for the survey of full-length transcript isoforms.  Barcoding system is available for multiplexed sequencing on a single SMRT cell to achieve time- and cost- effectiveness.

The Pacbio Sequel system has been installed at CGS in June 2017.  Protocols are being actively developed.  Please contact us if you are interested!

 

Applications:

Sample Type Service Type
DNA   Whole Genome Sequencing
DNA   Microbial Genome Sequencing
DNA   Targeted Sequencing
DNA   Metagenomic Sequencing
RNA   RNA Sequencing (Iso-seq)
 

Contact

  Mr Chengwei Wu
  Ms Tiky Leung
  Mr Clement Chow
  Mr Allen Chu

  Telephone: 2831 5478 / 2831 5477
  Email: thirdgenseq.cgs@hku.hk