Home Technology Seminars

Technology Seminar (27 Aug 2007)

Print
Technology Seminar

Sample preparation and fractionation for 2-D gels: if you cannot solubilize, you cannot analyse

 

By

 

Ben Herbert
Proteomics Technology Centre of Expertise
University of Technology, Sydney
This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

on Monday, 27 August 2007 at 10:30a.m.

Room L6-32, 6/F, Laboratory Block
Genome Research Centre
21 Sassoon Road, Pokfulam, Hong Kong
Abstract:

In the last 10 years much has changed in the science of sample preparation. On the other hand, the art of 2-D gels is almost the same, and remains a method that many love to hate. In reality, 2-D gels provide unparalleled protein resolution and enable a frozen-in-time view of the proteome. Importantly, this includes differentially modified and processed versions of many proteins.
One of the key reasons that researchers dislike 2-D gels is the difficulty of good sample preparation. As a consequence, poor sample preparation is the cause of most poor-quality 2-D gels. To get the best from 2-D gels, one must understand the sample that is being studied, know how to extract the desired proteins and prepare them for the 2-D gel in the most appropriate way. If you cannot solubilize, you cannot analyse! This can be a daunting challenge as the protein diversity within even the simplest proteome cannot be captured by any single extraction and separation step, and consequently the wealth of literature on sample preparation is remarkably diverse.
This seminar will be focused on simple methods that can deliver excellent results with many samples. The keys to high quality 2-D gels are good protein solubility and efficient removal of contaminants. Our lab has refined a small number of methods that work with most common contaminants and enable high quality 2-D gels. In addition, I will outline more advanced methods that enable membrane and low abundance proteins to be detected.

ALL ARE WELCOME
Co-organized with Bio-Rad Pacific Ltd

For enquiries, please call 2819-9848 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it