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Technology Seminar (6 Jul 2007)

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Technology Seminar

Quantitative Two-color Protein Detection Using the LI-COR Odyssey System

 

By

 

Michael van Waes, Ph.D.
Senior Field Applications Scientist
LI-COR Biosciences

 

on Friday, 6 July 2007 at 2:30p.m.

Room L6-32, 6/F, Laboratory Block
Genome Research Centre
21 Sassoon Road, Pokfulam, Hong Kong
Abstract:

Western Blotting has been the standard for protein detection for almost 30 years. The LI-COR Odyssey System adds the ability to quantify proteins on a Western Blot by using direct fluorescence detection without compromising sensitivity. In addition, each band can be normalized relative to a loading control or housekeeping protein, taking advantage of simultaneous two-color detection. The use of near infrared fluorophores ensures low background and great flexibility of applications, including immunofluorescence in 96- or 384-well format (In-Cell Western), In-gel immunostaining, immunohistochemistry, and many others.

Biography:

Dr. van Waes received his Ph.D. in Biochemistry from The University of Montana in 1997, studying the structure and function of the bacterial ribosome. He acquired experience in the field of fluorescence spectroscopy as a postdoctoral associate at Texas A&M University, and went on to a Faculty position at the University of Nebraska Medical Center. He has been with LI-COR since 2004 where he presently provides and coordinates support in the Asia-Pacific region and South America.

ALL ARE WELCOME
Co-organized with Gene Company Ltd.

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