Technology Seminar (30 Oct 2008)



Technology Seminar

Moving from in vitro to in vivo RNAi




Christofer CUNNING, Ph.D
Product Manager, RNAi Technology
Invitrogen Corporation

on Thursday, 30 October 2008 at 11:00 a.m.

Room L6-32, 6/F, Laboratory Block
Genome Research Centre
21 Sassoon Road, Pokfulam, Hong Kong


RNA interference (RNAi) is a powerful tool to inhibit gene expression and has revolutionized biology. The ability to demonstrate phenotypic alterations in animals represents the ultimate confirmation of conclusions arrived at in vitro, making a move to in vivo applications the logical progression for RNAi research. We have developed Stealthâ„¢ RNAi, a chemically-modified RNAi duplex, that displays increased specificity and enhanced stability in serum compared to standard siRNA. The advantages of Stealthâ„¢ RNAi chemistry combined with rational design based on knowledge of the target gene sequence are the ideal reagent for systematic inhibition of gene expression both in vitro and in vivo. Additionally, the development of a novel delivery reagent for in vivo RNAi will be highlighted. This delivery reagent enables successful in vivo RNAi in multiple organs. Here, we demonstrate the key components for successful RNAi and the experimental steps needed to move RNAi research in vivo.

Co-organized with Invitrogen Hong Kong Limited .