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Technology Seminar (14 Nov 2008)



Technology Seminar

Expanding the Range of Sequencing Applications
Using the SOLiD System



Robert Nutter
Sr. Staff Applications Scientist, Applied Biosystems

on Friday, 14 November 2008 at 2:30 p.m.

L6-32, 6/F, Laboratory Block
Li Ka Shing Faculty of Medicine Building
21 Sassoon Road, Pokfulam, Hong Kong


Next generation sequencing technologies allow researchers to adopt a systems-biology approach to studying the genomes, transcriptomes, and epigenomes of simple and complex organisms.   In order to facilitate this level of integrated science, the Applied Biosystems SOLiDâ„¢ System has rapidly developed into a platform providing unsurpassed flexibility, sequence throughput and accuracy. In collaboration with a growing number of customers, great progress has also been made in the development of end-to-end workflows (library preparation through data analysis) for a number of applications using sequence information.  The SOLiDâ„¢ 3 System, which will be release in early 2009, will provide the option of 50 bp single reads or 2X50 bp mate pair reads as well as the generation of  275 - 400 million sequences from one run.  This number of sequences combined with molecular barcoding enables the multiplexing of many samples, thereby dramatically reducing the cost/sample and enabling the efficient utilization of the sequencing capacity of the system.  We will present the latest results on genomic applications such as SNP discovery, structural variation and de novo sequencing; epigenomic applications such as ChIP-Seq, Methylation Patterns and transcriptomic applications such as miRNA, mRNA from single cells and whole transcriptome analysis.

Co-organized with Applied Biosystems Hong Kong Limited
Refreshment to be served