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Technology Seminar (1 Apr 2009)

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Technology Seminar

on Wednesday, 1 April 2009, at 10:30 a.m.

Seminar Room 4 (LG-S4), G/F, Laboratory Block,
Faculty of Medicine Building,
21 Sassoon Road, Pokfulam, Hong Kong

Part 1:   High-Definition Targeted Analysis of Human Disease with 2.1 Million Feature Microarrays
Speaker:   Dr. Xinmin Zhang
Roche NimbleGen, Inc.
Abstract:   Roche NimbleGen HD2 arrays give researchers total flexibility to utilize 2.1 million long probes to investigate mechanisms and identify genetic markers for human diseases. The HD2 arrays have been shown to offer superior performance in applications including CGH,  DNA Methylation, ChIP-chip, CGH and Gene Expression studies. With the high definition arrays, researchers are now beginning to make biomedical discoveries with unprecedented level of details and scale.
Most recently, an optimized HD2 exome array is also available for genomic selection and targeted sequencing of the entire human consensus coding sequence (CCDS) database, targeting ~180,000 coding exons from ~18,000 genes, totaling ~34 Mb of genomic sequence. This array technology, coupled to 454 sequencing technology, has been used to effectively sequence the exons of patient and normal DNA to identify disease-specific somatic mutations. Human whole exome sequencing enables researchers to investigate the complete coding portion of an individual genome rapidly, thus will have a huge impact on the future of biomedical research and personalized medicine.
     
Part 2:   Recent Advances in 454 Sequencing - Latest Developments of Technology and New Applications
Speaker :   Dr. Guido Kopal
Roche Applied Science, Scientific Consultant Genome Sequencing
Abstract:   454 Sequencing combines high throughput sequencing with the unique benefits of long reads and exceptional accuracy. Many high profile studies have been carried out using the technology. Outstanding performance has been demonstrated in different applications including de novo sequencing, re-sequencing, and metagenomics.. For example, 454 Sequencing has been used in detecting very low frequency somatic mutations linked to cancer. Also, sequencing of highly polymorphic regions like HIV genomes and the human HLA locus has been successfully addressed by utra-deep amplicon sequencing. 
This presentation will provide a short overview about the 454 Sequencing technology, and present results of studies in all major areas of applications, from de novo and transcriptome analysis to metagenomic sequencing. Details about new technology development including the recent launch of Titanium kits capable of generating 500 megabases per run (5 gigabases per week) at an average read length of ~400 bases will also be covered.

ALL ARE WELCOME

Co-organized with Roche Diagnostics (HK) Ltd.
Refreshment to be served

For enquiries, please call 2819-9842 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it .