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Technology Seminar (12 May 2009)

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Technology Seminar


12 May 2009 (Tue)   10:00am - 12:00noon



Room L6-32, 6/F, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong

 

Speaker:

Dr. Ray Meng
International Field Application Specialist
Gene Expression Division, Bio-Rad USA

 
 

Part 1:

The Most Out of Your Real-time Experiments

 
 
Abstract:

While real-time RT-PCR has a tremendous potential for analytical and quantitative applications, a comprehensive understanding of its underlying principles is important. In this talk, experts in the field will present a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique. Topics include assay design, primer design, temperature ramping rate, reaction efficiency, fast real time PCR, relative quantification: important parameters for successful experiments.

 
 

Part 2:

High Resolution Melt (HRM) – the Theory, Applications + Demo

 
 
Abstract:

When it comes to genotyping and mutation scanning, high-resolution DNA melting is emerging as the technique of choice because it is inexpensive, simple, accurate and rapid. Development of this method of DNA analysis has been underway since its introduction in 2002 by a team of researchers from Pathology Department led by Dr. Carl Wittwer and Dr. Karl Voelkerding at the University of Utah coupled with collaborative efforts from Idaho Technology.

In this seminar, the fundamentals, applications, and the workflow (including assay design and data analysis, etc.) using Bio-Rad Real-time qPCR instrument, reagent, and software will be introduced.

 

ALL ARE WELCOME


Co-organized with Bio-Rad
Refreshment to be served
For enquiries, please call 2819-9842 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it