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Technology Seminar (29 May 2009)

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Technology Seminar



EpiTect - Setting Standards in Epigenetics

Novel Technologies Offering a Complete Workflow for Reliable

Detection and Accurate Quantification of DNA Methylation


By

Dr. Gerald Schock

Senior Global Product Manager Epigenetics




29 May 2009 (Fri)


11:00am - 12:00noon



Room L6-32, 6/F, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong


 
 

Abstract:

Epigenetics, one of the fastest growing areas in molecular biology, encompasses study of DNA methylation and chromatin remodelling. Transcriptional silencing associated with DNA methylation is required for fundamental processes e.g. embryonic development, intragenomic parasites protection, female X-inactivation, genomic imprinting, and cognitive functions. Moreover, aberrant promoter methylation in tumor suppressor genes has emerged as a major cause leading to cancer.

Both location and frequency of DNA methylation appear significant, which presents a challenge as researchers often focus on subtle changes in methylation patterns. Standardized tools are necessary for reliable results and accurate analysis.

This presentation will review latest DNA methylation analysis and highlight critical procedures including complete conversion of unmethylated cytosine and avoidance of DNA degradation.

We will also describe methods to analyze bisulfite-converted DNA e.g. TaqMan® probe-based real-time PCR (MethyLight PCR/ assays), HRM (High-Resolution-Melting), and quantitative sequencing. Finally, applications of these methods at different stages of research will be discussed.

 


ALL ARE WELCOME


Co-organized with QIAGEN Hong Kong Pte. Ltd.
Refreshment to be served
For enquiries, please call 2819-9842 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it