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Technology Seminar (5 Jun 2009)



Technology Seminar

Imaging of Chemiluminescent Western Blots:

Comparison of Digital Imaging and X-ray Film


Mr. Anthony A. Alburo

Product Manager, Bio Rad Laboratories, USA

5 June 2009 (Fri)

10:30 - 11:30 am

Room L6-32, 6/F, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong



Western blotting is a widely used procedure that uses antibodies to detect and quantify a specific protein in a complex mixture. Proteins separated by polyacrylamide gel electrophoresis are transferred onto a membrane before probing with a specific antibody. The membrane-bound antibody-protein complex is detected by a secondary antibody conjugated to horseradish peroxidase (HRP), in conjuction with a substrate that produces a luminescent signal.

Traditionally, the chemiluminescent signal is detected by exposure of the membrane to a light-sensitive film. The recent development of CCD based digital imaging systems that capture the luminescent signals on western blots has provided new options for the detection and quantitation of signals. A comparison of the performance of X-ray films to Bio-Rad’s digital imaging systems with regard to the visualization and quantitation of chemiluminescent signals on western blots reveals additional values that extend the data offered by western blotting.



Co-organized with Bio-Rad
Refreshment to be served
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