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Technology Seminar (5 Jun 2009)

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Technology Seminar


Imaging of Chemiluminescent Western Blots:

Comparison of Digital Imaging and X-ray Film

By

Mr. Anthony A. Alburo

Product Manager, Bio Rad Laboratories, USA
 


5 June 2009 (Fri)

10:30 - 11:30 am




Room L6-32, 6/F, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong

 
 

Abstract:

Western blotting is a widely used procedure that uses antibodies to detect and quantify a specific protein in a complex mixture. Proteins separated by polyacrylamide gel electrophoresis are transferred onto a membrane before probing with a specific antibody. The membrane-bound antibody-protein complex is detected by a secondary antibody conjugated to horseradish peroxidase (HRP), in conjuction with a substrate that produces a luminescent signal.

Traditionally, the chemiluminescent signal is detected by exposure of the membrane to a light-sensitive film. The recent development of CCD based digital imaging systems that capture the luminescent signals on western blots has provided new options for the detection and quantitation of signals. A comparison of the performance of X-ray films to Bio-Rad’s digital imaging systems with regard to the visualization and quantitation of chemiluminescent signals on western blots reveals additional values that extend the data offered by western blotting.

 


ALL ARE WELCOME


Co-organized with Bio-Rad
Refreshment to be served
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