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Technical Seminar (20 May 2010)



Technology Seminar

Analysis of Cytokine Profiles to Define Cell Lineages and Patterns Linked to Different Disease Using Different Immunological Assays


Dr. John Wijdenes

General Manager & Scientific Director
Gen-Probe Diaclone

20 May 2010 (Thu)

4:00 – 5:00 pm

Room L6-32, 6/F, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong



After Mosmann divided mouse CD4+ T helper cells into two distinct populations, at least 8 new T cell populations have been described.

The cytokine production profile has become the generally accepted signature of a particular T cell population. In this regard, the cytokine network represents a signaling system with a substantial functional redundancy, conferring a remarkable stability to, and allowing dynamic responses of the immune system. Using multiplex cytokine analysis the results suggest that the healthy and biased states of the cytokine network can be defined, and as such it may serve as a potential meta-biomarker for differentiation between autoimmune and inflammatory diseases.

Also, the Elispot assay, together with the ELISA and Cytoplasm staining will help clarifying the situation. All four technologies help to create a cytokine profile, differentiate between cytoplasm storage and/or secretion, extend to time to measure secretion and tell per cell what is secreted and how much.



Kind Reminder: Please take off your lab coat before coming to the seminar.

Co-organized with Genetimes Technology Int’l Holding Ltd.
Refreshment to be served
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