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Technology Seminar (16 Sep 2010)



Technology Seminar

A Practical Approach to Assay Design for qPCR-Overcoming Difficult Assays, Designs and Optimizations while Conforming to the MIQE Guidelines


Prof Stephen Bustin

University of Middlesex, London

16 September 2010 (Thu)

14:00 pm – 15:30 pm

Room L6-32, 6/F, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong



Quantitative PCR (qPCR) assays have often been run without serious specificity and quality considerations. Target sequence information is usually inserted into “black-box” software packages and most assays are run under general conditions. With the arrival of MIQE guidelines, a greater emphasis is placed on the use of robust and validated assays. Generating robust assays is generally a simple task, but occasionally obstacles can be encountered. Ease in the optimization of an assay is often directly linked to the original design. In this talk, we will discuss the critical assay design parameters and tips to overcome potential problems, as well as quick optimization and validation strategies that help qPCR assays satisfy MIQE guidelines.



Kind Reminder: Please take off your lab coat before coming to the seminar.

Co-organized with Bio-Rad
Refreshment to be served

For enquiries, please call 2819-9842 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it