Technology Seminar (30 May 2011)



Technology Seminar

The Use of Next Generation Sequencing and Sequence Capture to Study Human Genome Variation and Cancer


Dr. Bernd Timmermann

Head, Next Generation Sequencing Group,
Max Planck Institute

30 May 2011 (Mon)

4:00 – 5:00pm

Seminar Room 4, Lower Ground Floor, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong



In an IRON (Interlaboratory RObustness of Next-Generation Sequencing) study we investigated - as an international consortium - the robustness, precision, and reproducibility of 454 amplicon sequencing across 10 laboratories from 8 countries. We demonstrated that the method achieves a high concordance across multiple laboratories, and allows a broad and in-depth molecular characterization of hematological malignancies. However more comprehensive experimental approaches are needed to identify novel cancer genes, and potentially disease-causing variants. To this end, we have begun "whole exome" sequencing of colon cancer patients using sequence capture of ~180,000 coding regions followed by massively parallel sequencing. The challenge of analyzing the data is handled by our automated analysis pipeline.



Kind Reminder: Please take off your lab coat before coming to the seminar.

Co-organized with Roche Diagnostics (Hong Kong) Ltd.
Refreshment to be served

For enquiries, please call 2819-9848 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it