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Technology Seminar (27 June 2011)

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Technology Seminar

ArcturusXT Laser Capture Microdissection System: Optimized Protocol for Laser dissection of living cells and Microgenomics

By


Dr. Amy Lam

Field Application Scientist



27 June 2011 (Mon)

3:00 – 4:00pm



Room L6-32, 6/F, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong

 
 

Abstract:

Laser capture microdissection (LCM) is a proven technique for isolating pure cell populations for downstream molecular analysis. The combination of UV laser cutting and non-damaging infrared (IR) LCM permits rapid and precise isolation of larger numbers of cells while maintaining cellular and nucleic acid integrity necessary for downstream analysis.

It is challenging to utilize pure cell populations obtained through LCM for microarray analysis which usually limited to several micrograms of total RNA for hybridizations. The Applied Biosystems® ArcturusXT LCM system provides a complete and robust solution for microarray analysis in Microgenomics, including microdissecting pure cell populations from tissue biopsies, isolating high-quality RNA from microscopic samples, amplifying picogram amounts of total RNA isolated from frozen tissue, and non-enzymatic labeling of amplified antisense RNA (aRNA) for microarray hybridizations. This integrated platform is incorporated with optimized protocols to allow analysis of cells at various stages.

 


ALL ARE WELCOME

Kind Reminder: Please take off your lab coat before coming to the seminar.


Co-organized with Applied Biosystems HK Ltd
Refreshment to be served

For enquiries, please call 2819-9848 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it