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Technology Seminar (29 June 2011)

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Technology Seminar

Solutions to Eradicates Stem Cell Culture Obstacles & Barriers Using Divers Surface Coating Technology

By


Ms. Hyunju Lee

Thermo Fisher Scientific


29 June 2011 (Wed)

10:00am – 12:00pm



Room L6-32, 6/F, Laboratory Block

Li Ka Shing Faculty of Medicine Building

21 Sassoon Road, Pokfulam, Hong Kong

 
 

Abstract:

Advanced and innovative cell culture tools can help you achieve excellence at every stage of your stem cell culture process.
The concerns over contaminants from animal components and batch-to-batch variability of the coating matrices for human stem cells culture have significantly hindered the usage of these types of surfaces for translational and clinical applications. The Thermo Scientific Nunclon Vita is an energy-treated polystyrene surface free of animal components. It enables culture of human stem cells without matrix or feeder layers. Human embryonic stem (ES) cells are grown directly on the surface in conditioned media containing ROCK-inhibitor, and can be sustained for more than ten passages without signs of differentiation.
Secondly during the session we will present novel temperature responsive surface, ‘Upcell’. Upcell can completely abolish the use of cell damaging proteolytic enzymes such as trypsin. Moreover, when cells are cultured beyond over-confluency, the cells can be collected in a form of thin-film, so-called as "cell-sheet" and it also allows the creation of 3D tissue construct when we stack the cell –sheet.

 


ALL ARE WELCOME

Kind Reminder: Please take off your lab coat before coming to the seminar.


Co-organized with Kou Hing Hong Scientific Supplies Ltd.
Refreshment to be served

For enquiries, please call 2819-9848 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it