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Technology Seminar (16 May 2008)

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Technology Seminar

Next-Generation DNA Sequencing – Recent Advances & New Applications

by

PATRICK NG
Scientific Liaison Manager, Asia-Pacific
Roche Diagnostics Asia-Pacific Pte Ltd


3:30 p.m., May 16th, 2008 (Friday)

Seminar Room 4, G/F, Laboratory Block,
Li Ka Shing Faculty of Medicine Building
21 Sassoon Road, Pokfulam, Hong Kong

Abstract:
The Roche-454 GS-FLX genome sequencer is a second-generation, massively parallel high-throughput sequencing platform. Based on enhanced pyrosequencing technology, the GS-FLX differs from other sequencing platforms in its unique combination of high multiplexity (>400,000 reads per run), extraordinarily long read length (~250 bases per read), high accuracy (99.5% single-run; >99.995% consensus) and short run-time (~8 hours per run). Kit improvements scheduled for release later this year will result in even greater multiplexity and increased read lengths, on the same basic machine platform. More than 140 articles using this sequencing platform have been published in leading scientific journals since its introduction in 2005, covering a wide spectrum of applications from de novo genome sequencing and assembly, to genome-wide structural variation identification using paired-end mapping. More recently, the introduction of Roche-NimbleGen Sequence Capture (SeqCap) custom microarrays provides, for the first time, a commercial solution for researchers interested in targeted resequencing of defined genomic loci up to several millions of basepairs in size. The purpose of this talk is to give a brief introduction to the GS-FLX sequencing technology, and a sampling of the many applications that are possible using this systems.

Co-organized with Roche Diagnostics (HK) Ltd