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Technology Seminar (19 Jun 2008)

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Technology Seminar

Stem cell gene expression profiling and copy number
variation (CNV) analysis using a novel, ultrasensitive
and highly versatile microfluidic platform

By

Robert C. Jones
Executive Vice President of Research &
Development of Fluidigm

on Thursday, 19 June 2008 at 10:00 a.m.

Room L6-32, 6/F, Laboratory Block
Genome Research Centre
21 Sassoon Road, Pokfulam, Hong Kong
Abstract:

Historically, single-cell gene expression experiments have been difficult and expensive to perform. Fluidigm has introduced a new single-cell gene expression technique that, when used with the BioMarkTM Real-Time PCR System, produces inexpensive, reproducible, gene expression results from single-cell samples.
The method is ideally suited for high-throughput cell-line studies to determine individual cell behavior in what has been believed to be homogeneous populations. The method is also well suited to determine single gene cell expression levels in circulating tumor cells (CTCs) and to differentiate stem cells.
The single-cell gene expression technique can be used with either the BioMarkTM 48.48 Dynamic Array or the 96.96 Dynamic Array to examine up to 96 genes per individual cell and 96 cells at a time. The technique uses off-the-shelf TaqMan reagents and performs real-time qPCR of the same quality you have come to expect from traditional cDNA microtiter plate experiments.
Current methods to analyze copy number variation (CNVs) mainly depend on SNP based microarrays or comparative genomic hybridization and are therefore subject to low sensitivity and low resolution. Real-time PCR systems based on microwell plates are also limited in that they do not reliably distinguish less than two-fold differences in copy number. The BioMarkTM 12.765 Digital Array enables a new level of sensitivity and precision in detecting copy number variations.
Discover how the BioMarkTM system can help you to efficiently sort through the multitude of possibilities and find the right answers. We invite you to get acquainted with BioMarkTM applications, chips and instrumentation.

ALL ARE WELCOME
Co-organized with Bio-Gene Technology Ltd

For enquiries, please call 2819-9848 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it