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Technology Seminar (21 Sep 2012)

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Technology Seminar

Multiplex Digital Nucleic Acid Quantitation Using Molecular Barcodes

By

Mr. Paul Rasmussen

Regional Manager and Field Applications Scientist



21 September 2012 (Friday)

2:00 – 3:00pm



Room 7-03, 7/F,

The Hong Kong Jockey Club Building for Interdisciplinary Research

5 Sassoon Road, Pokfulam, Hong Kong


 

Abstract:

The NanoString nCounter system is a novel platform for direct digital quantification of nucleic acids, including difficult samples such as cell, tissue, and blood lysates, as well as RNA and DNA from FFPE tissues. The multiplexing capacity of 10s to as many as 800 different genes / targets in a single reaction facilitates screening of complex signatures, pathways, or gene classes with incredible simplicity and precision. The technology works by directly capturing, imaging, and counting fluorescent barcodes tagged to individual nucleic acid molecules. This is done without amplification or reverse transcription (partially explaining the success of the platform with lysates and degraded samples). The result is an assay capable of sensitive and precise quantification of mRNA, miRNA, or DNA (for CNV or ChIP screening), as well as the characterization of gene fusions, splice variants, and even microbial populations. An introduction to the assay chemistry and performance will be provided, followed by specific examples of the application of the technology.

 

ALL ARE WELCOME

Kind Reminder: Please take off your lab coat before coming to the seminar.


Co-organized with Advanced TNI Development (HK) Co. Advanced TNI Logo

Refreshment to be served

For enquiries, please call 2831-5500 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it