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Technology Seminar (4 Nov 2014)

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Technology Seminar

Applications of PacBio Single Molecule,
Real-Time (SMRT) DNA Sequencing

By

Dr Stephen Turner
CTO
Pacific Biosciences


4 November 2014 (Tuesday)
11:00am – 12:00pm



Seminar Room 1B, G/F
The Hong Kong Jockey Club Building for Interdisciplinary Research
5 Sassoon Road, Pokfulam, Hong Kong
 

Abstract:

Genomic data have become commonplace in most branches of the biological sciences and have fundamentally altered the way research is conducted.
However, the predominance of high-throughput, short-read sequence data from second-generation technologies has commonly resulted in fragmented and partial genomic and transcriptomic data, limiting the type of genetic variation able to be characterized. Long, unbiased reads from SMRT sequencing now allow for a return to more contiguous and comprehensive views of genomes and transcriptomes.
I will present several examples highlighting this transition, including improved de novo genome assemblies, characterizing structural genetic diversity, and resolving previously unsequenceable genomic regions. In the area of RNA-seq, I will highlight numerous examples of full-length transcript recall and splice isoform characterization in human and other complex transcriptomes that demonstrate that the identification of transcript isoforms and even gene content is far from complete.

 

ALL ARE WELCOME


Co-organized with Gene Company  Gene Company Logo
Refreshment to be served
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