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Technology Seminar (5 Dec 2014)

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Technology Seminar

Single-cell RNA Sequencing

By

Dr Andrew Alan Farmer
Vice President, Research & Development
Clontech Laboratories, Inc.


5 December 2014 (Friday)
3:00 – 4:00pm



Seminar Room 1A, G/F
The Hong Kong Jockey Club Building for Interdisciplinary Research
5 Sassoon Road, Pokfulam, Hong Kong
 

Abstract:

Next Generation Sequencing (NGS) has increased our understanding of biology by enabling highly sensitive RNA expression analysis throughout the transcriptome, across a wide dynamic range. Two powerful applications, single cell RNA-seq and stranded RNA-seq, have been the focus of considerable efforts in protocol innovation. By utilizing the template switching activity of reverse transcriptase, Clontech’s patented SMART™ technology has enabled researchers to analyze their most challenging samples. The dT-primed SMART-based RNA-seq protocol, designed to work with high-quality RNA or whole cells, is the gold standard for single cell analysis. This technology has also been adapted for transcriptome analysis for the Fluidigm C1 platform. SMART’s applicability has recently been extended to noncoding RNA and mRNA from degraded samples. The purpose of this seminar is to take a deeper look into new approaches required to study genomics and transcriptomics at the single- cell level, and technologies that are being developed to do so.

 

ALL ARE WELCOME


Co-organized with Bio-Gene Technology Ltd.Bio-Gene Logo
Refreshment to be served
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