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Technology Seminar (20 May 2015)



Technology Seminar

Genome Editing Solution:
New Workflow Tools for Editing Genomes


Dr Jian-Ping Yang
Staff Scientist

20 May 2015 (Wed)
10:30 – 11:30am

Seminar Room 1A, G/F
The Hong Kong Jockey Club Building for Interdisciplinary Research
5 Sassoon Road, Pokfulam, Hong Kong


Genome editing with engineered nucleases, including zinc-finger nuclease (ZFN), transcriptional activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPRs), is a transformative technology for efficiently modifying any genomic sequence of interest. Understanding the workflow, and probing the efficiency and consequences of gene editing are crucial for successful precision genome manipulation. We have developed various genome editing tools that enable and simplify a broad range of genome editing cell engineering applications, including the development of knock-in, knockout and transgenic organisms.

The presentation will cover the following tools and workflows:

  • Genome editing workflow
  • CRISPR design
  • Detection and analysis methods for gene modification
  • Methods for selectively enriching engineered nuclease-modified cells
  • Utilizing Cas9 protein or Cas9 mRNA
  • High efficiencies for bi-allelic multiplex knock outs
  • Lentiviral CRISPR libraries


Co-organized with Life Technologies 
Refreshment to be served
For enquiries, please call 2831-5500 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it