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Technology Seminar (11 Sep 2015)

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Technology Seminar

NuGEN: Innovative Sample Prep Solutions
for Next-Generation Sequencing

By

Dr Oliver Vasilevski
Director of Sales - Asia Pacific


11 September 2015 (Fri)
10:00 – 11:30 am



Seminar Room 4, 4/F
The Hong Kong Jockey Club Building for Interdisciplinary Research
5 Sassoon Road, Pokfulam, Hong Kong
 

Abstract:

This talk will describe a novel method for RNA-Seq library construction which utilizes Insert Dependent Adapter Cleavage (InDA-C), for effective removal of specific transcripts from RNA-Seq libraries without impacting non-targeted transcripts. InDA-C employs specific and robust enzymatic steps to eliminate undesirable transcripts such as rRNA during library construction without perturbing the original total RNA population as with hybridization capture methods. The specificity of transcript depletion relies on InDA-C primers which can be designed to target virtually any class of unwanted transcripts from any species. The library construction workflow uses as little as 10 ng of input total RNA, produces a strand-specific library, and is highly adaptable for depletion of any unwanted transcript(s). Through the use of InDA-C technology a greater percentage of RNA-Seq sequencing reads can be directed towards desired coding and non-coding transcripts.

Speaker:

Oliver Vasilevski received his PhD in Molecular Biology and Biochemistry for his work on the underlying molecular mechanisms governing cardiac hypertrophy at the Baker Heart Research Institute in Melbourne, Australia. After holding several academic research and teaching position he joined Millennium Science as a Field Applications Scientist and later as a Product Manager of the Genomics portfolio which amongst others included NuGEN. Following his time at Millennium Science, Oliver joined Illumina as the Asia Pacific Senior Market Development Manager responsible for developing the genotyping market throughout Asia and then was the Senior Channel Manager for Asia Pacific at Fluidigm. Oliver is now the Director of Sales for the Asia Pacific Region at NuGEN.

 

ALL ARE WELCOME


Co-organized with Genetimes Technology Inc. Genetimes Technology Inc Logo
Refreshment to be served

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