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Technology Seminar (28 Jun 2016)

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Technology Seminar

Validation of NGS and RNA-seq Data by qPCR

Technologies

By

Dr Sunny Wong

Roche Diagnostics Hong Kong

28 June 2016 (Tue)

11 am – 12 noon

Seminar Room 4-02, 4/F

The Hong Kong Jockey Club Building for Interdisciplinary Research 

5 Sassoon Road, Pokfulam, Hong Kong

 

 
 

Abstract:

Next-generation sequencing (NGS) has allowed the generation of huge amount of genome data, making data validation at times challenging. Real-time quantitative PCR (qPCR) is well-adopted in many laboratories and is accepted as the gold standard for assessing gene expression level.  With advancement in instrument design and protocol development, qPCR has also emerged as a powerful tool for downstream NGS data validation. In this seminar, three applications will be highlighted:

(1)  Melt-curve genotyping facilitates NGS data validation and complicated mutation studies.

(2) High-resolution melting (HMR) allows fast and accurate assessment of mutation analysis that is potentially applicable for validating NGS data

(3) Universal Probe Library offers a rapid, reliable and powerful tool for validating expression changes in potentially large number of gene targets from RNA-seq.

Speaker:

Our speaker Sunny Wong is the Applications Specialist from Roche Diagnostics Hong Kong. He has been with Roche in application supports of qPCR, nucleic acid purification and targeted sequencing.

 

ALL ARE WELCOME


Co-organized with Roche Diagnostics (Hong Kong) Limited 
Refreshment to be served

For enquiries, please call 2831-5500 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it