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Technology Seminar (1 Nov 2016)

Technology Seminar

Mr Yann Lecouturier

 Dolomite Bio Regional Director Europe and China


Ms Nadia Shakir

Dolomite Bio Applications Scientist

1 Nov 2016 (Tue)

 10:45 am – 12:30 pm

Introduction to Droplet Mircofluidics: Principles and Benefits

with on-site demonstration (Laboratory Room 5-01, 5/F, HKJCBIR)

1:30 – 3:45 pm

Introduction and Discussion on Single Cell RNA-Seq

with on-site demonstration (Laboratory Room 5-01, 5/F, HKJCBIR)

Seminar Room 7-03, 7/F

The Hong Kong Jockey Club Building for Interdisciplinary Research

5 Sassoon Road, Pokfulam, Hong Kong




Traditional methods of single cell isolation can be highly laborious and tricky.In this seminar we will present a simple microfluidic pipeline which encapsulate cells into picolitre volume droplets, and enables isolation of thousands of single cells in a short period of time. We will provide an overview of the droplet microfluidics and discuss how this can cater biological applications such as RNA-seq, profiling of T cell receptors, isolating antibody coding sequences, and producing droplets for FACS sorting libraries. We will also address how to carry out these applications using our systems and explain the benefits of using microfluidics. Special focus will be on RNA-Seq application and demonstration will be provided during the practical session.


About the Speaker:
Mr Yann Lecouturier
Yann studied Chemistry, Maths and Physics for 4 years at the University in Marseille, France. In 2001, he joined the prestigious “Ecole d’Ingénieur de Chimie de Montpellier” where he specialized for another 2 years in Organic Chemistry, Medicinal and Biochemistry. Yann worked in Spain and Paris before joining Pfizer UK in 2004. Yann worked as a Medicinal chemist for 4 years as well as Process Development Chemist for one year at Pfizer. In 2009, Yann had a career change and moved to Dolomite initially as a technical specialist but moved very rapidly into a commercial role. Yann is now Regional Director of Europe and China.

Ms Nadia Shakir
Nadia studied human biology at the University of Worcester, and then went on to study Cancer Sciences for her Masters by Research. She then joined Dolomite in 2015 as a Graduate Trainee for 6 months and then became an Application Scientist for Dolomite Bio. She has spent a lot of her time developing the current single cell systems with the rest of the biology team in the UK and working with customers on installations and training worldwide.


Co-organized with UATIL and Dolomite Bio   UATIL Logo  Dolomite Bio Logo

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