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Technology Seminar (04 Sep 2017)

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Technology Seminar

Technologies for High Throughput Single Cell Gene Expression, Immune Repertoire Profiling and Long Range Sequence Information Using Short Read Sequencers


 Mr Joseph Aman

Field Application Specialist, 10x Genomics


4 Sep 2017 (Mon)

10am – 11am  


Seminar Room 3, G/F
 

The Hong Kong Jockey Club Building for Interdisciplinary Research 

5 Sassoon Road, Pokfulam, Hong Kong

 

 

Abstract:
This seminar provides an introduction to the 10x Chromium System, which is capable of partitioning cells or HMW DNA into droplets, and preparing sequencing libraries in a highly parallel fashion such that all fragments produced in a single partition share a common barcode. The Chromium Single Cell applications allow both 3' gene expression profiling of hundreds to millions of cells, as well as the assembly of full-length V(D)J sequences on a cell-by-cell basis for detailed analysis of the adaptive immune system. On the DNA side, Chromium Genome kit employs Linked-Reads to fully resolve genic phasing, reveal structural variation and detect variants in previously inaccessible and complex regions of the genome. The talk will give an overview of all three applications and some of the research discoveries they are being used to unlock.

About the Speaker:
Joseph Aman started working in the field of NGS at the University of Southern California, California, in 2007, after they purchased one of the early Genome Analyzer instruments. During his time there, he was primarily focused on developing bisulfite and other epigenetic sequencing applications. In 2010, he left USC to work as an FAS for Illumina covering many of the key accounts in the Los Angeles area for both the array and sequencing platforms. In 2015, he joined 10x Genomics as an FAS and is now working with accounts in the APAC region.
 

ALL ARE WELCOME


Co-organized with 10x Genomics 10 Genomics Logo

Refreshment to be served
For enquiries, please call 2831-5500 or write to This e-mail address is being protected from spambots. You need JavaScript enabled to view it